Expanding the Repertoire of Natural Product-Inspired Ring Pairs for Molecular Recognition of DNA
نویسندگان
چکیده
A furan amino acid, inspired by the recently discovered proximicin natural products, was incorporated into the scaffold of a DNA-binding hairpin polyamide. While unpaired oligomers of 2,4-disubstituted furan amino acids show poor DNA-binding activity, furan (Fn) carboxamides paired with N-methylpyrrole (Py) and N-methylimidazole (Im) rings demonstrate excellent stabilization of duplex DNA as well as discrimination of noncognate sequences, consistent with function as a Py mimic according to the Py/Im polyamide pairing rules.
منابع مشابه
Expanding the repertoire of heterocycle ring pairs for programmable minor groove DNA recognition.
The discrimination of the four Watson-Crick base pairs by minor groove DNA-binding polyamides have been attributed to the specificity of three five-membered aromatic amino acid subunits, 1-methyl-1H-imidazole (Im), 1-methyl-1H-pyrrole (Py), and 3-hydroxy-1H-pyrrole (Hp) paired four different ways. The search for additional ring pairs that demonstrate DNA-sequence specificity has led us to a new...
متن کاملEvaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers
Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants. The study of the genetic diversity of violets as a native plant with ornamental and medicinal uses is the great importance in advancing the breeding goals of this plant. So...
متن کاملDNA Barcoding: a new tool with wide array of applications
DNA barcoding is a new term introduced in to scientific literatures by Hebert and coworkers almost a decade ago. The concept of barcoding alone is well-known to the public: a series of black bars printed on many commercial products (Universal Product Code), which are used to distinguish different products. Advances made in molecular biology and molecular techniques late 20th century e.g. sequen...
متن کاملMolecular Modeling of indeno [1,2-b] quinoline-9,11-diones as cytotoxic agents
Deoxyribonucleic acid (DNA) is an important molecular target for anti-cancer agents due to its involvement in gene expression and protein synthesis which are fundamental steps in cell division and growth. A number of antineoplastic agents interfere with DNA and hence disturb the cell cycle. Compounds including planar aromatic rings are privileged scaffolds in binding to the DNA. This characteri...
متن کاملA MODEL FOR THE BASIC HELIX- LOOPHELIX MOTIF AND ITS SEQUENCE SPECIFIC RECOGNITION OF DNA
A three dimensional model of the basic Helix-Loop-Helix motif and its sequence specific recognition of DNA is described. The basic-helix I is modeled as a continuous ?-helix because no ?-helix breaking residue is found between the basic region and the first helix. When the basic region of the two peptide monomers are aligned in the successive major groove of the cognate DNA, the hydrophobi...
متن کامل